5W83

Rpn8/Rpn11 dimer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	58.720, 74.990, 82.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.482 - 1.554
R-factor	0.2143
Rwork	0.213
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o8x
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	55.482
High resolution limit [Å]	1.550
Number of reflections	52965
<I/σ(I)>	13.1
Completeness [%]	99.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1.5 M ammonium tartrate pH 6.8