5W7X
Crystal Structure of FHA domain of human APLF in complex with XRCC1 bisphospho peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-22 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.119, 36.799, 98.284 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 25.497 - 2.005 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w7w |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.581 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.497 | 2.050 |
| High resolution limit [Å] | 2.005 | 2.005 |
| Rpim | 0.031 | 0.214 |
| Number of reflections | 28032 | 1098 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.9 | 75.3 |
| Redundancy | 4.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.6mM XRCC1 bisphosphopeptide 0.6mM APLF 0.5M lithium chloride 0.1M Tris 28% PEG 6000 |
