5W7T
STRUCTURE OF PHOSPHORYLATED WNK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | APEX II CCD |
| Wavelength(s) | 0.97112 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.175, 62.230, 120.899 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 30.660 - 2.010 |
| R-factor | 0.17528 |
| Rwork | 0.172 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FPQ |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.660 |
| High resolution limit [Å] | 2.010 |
| Number of reflections | 39997 |
| <I/σ(I)> | 7.7 |
| Completeness [%] | 94.2 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289.15 | 0.0425 M CITRIC ACID, 0.0575 M BIS TRIS PROPANE, 14% PEG 3,350, pH 6.0 |
