5W7Q
Crystal Structure of Reconstructed Bacterial Elongation Factor Node 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.476, 104.046, 43.833 |
| Unit cell angles | 90.00, 111.27, 90.00 |
Refinement procedure
| Resolution | 30.537 - 2.500 |
| R-factor | 0.2002 |
| Rwork | 0.197 |
| R-free | 0.24720 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.099 | 0.066 | 0.435 |
| Total number of observations | 47115 | ||
| Number of reflections | 10877 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 92.2 | 90 | 82.4 |
| Redundancy | 4.3 | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% Peg 10K, 100mM Na Hepes |
