5W7N

2-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	80.686, 37.690, 62.180
Unit cell angles	90.00, 95.95, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.19433
Rwork	0.192
R-free	0.23201
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g8u
RMSD bond length	0.020
RMSD bond angle	2.238
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.850	1.860
High resolution limit [Å]	1.800	1.800
Rpim	0.027	0.085
Number of reflections	17561	913
<I/σ(I)>	21.8	5.3
Completeness [%]	88.9	52.7
Redundancy	4.3
CC(1/2)		0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES pH 6.5, 12%(w/v) PEG 20000