5W6T

Crystal structure of the A/Puerto Rico/8/1934 (H1N1) influenza virus hemagglutinin in complex with cyclic peptide CP151070 (P7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0331
Spacegroup name	I 21 3
Unit cell lengths	164.331, 164.331, 164.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.080 - 2.590
R-factor	0.221
Rwork	0.219
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru7
RMSD bond length	0.009
RMSD bond angle	0.966
Data reduction software	HKL-2000 (712)
Data scaling software	HKL-2000 (712)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.630
High resolution limit [Å]	2.590	2.590
Number of reflections	21313
<I/σ(I)>	14.8	1.2
Completeness [%]	92.2	90.9
Redundancy	3.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	277	HA: 10 mg/ml 1M lithium chloride 20 % w/v PEG 6000 0.1M MES, pH=5.3