5W67
HLA-C*06:02 presenting VRSRR(ABA)LRL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.065, 81.575, 116.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.197 - 2.300 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nt6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.090 | 0.420 |
| Number of reflections | 19189 | 6072 |
| <I/σ(I)> | 7.5 | 3.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1M Na acetate pH 4.5 and 1.8 M ammonium citrate |
