5W5N
Crystal structure of human IgG4-Sigma2 Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-17 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.520, 78.470, 97.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.264 - 1.850 |
| R-factor | 0.1827 |
| Rwork | 0.181 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (dev_1428) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.264 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.056 | 0.670 |
| Number of reflections | 49481 | 3648 |
| <I/σ(I)> | 19.82 | 3.02 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.35 | 7.42 |
| CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% (w/v) PEG 20K, 0.1 M sodium acetate, 5.5, 5% (v/v) MPD |
