5W5L
Crystal structure of human IgG1-Sigma Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.360, 79.170, 101.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.282 - 1.900 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ave |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.213 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (dev_1428) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.282 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.041 | 0.539 |
| Number of reflections | 47175 | 3427 |
| <I/σ(I)> | 21.27 | 2.93 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.4 | 5.7 |
| CC(1/2) | 0.999 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 9% PEG 20,000, 0.1 M Sodium Acetate, pH 5.5 |
