5W5I

Human IFIT1 dimer with PPP-AAAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	94
Detector technology	CCD
Collection date	2015-06-16
Detector	RAYONIX MX300HE
Wavelength(s)	0.979
Spacegroup name	I 4
Unit cell lengths	184.789, 184.789, 88.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.397 - 2.650
R-factor	0.22
Rwork	0.219
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.477
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rpim	0.025	0.383
Number of reflections	43686
<I/σ(I)>	29.6	2
Completeness [%]	99.8	99.2
Redundancy	6.4	6.4
CC(1/2)		0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	277.15	27 - 32 % PEG 200, 0.1 M Tris pH 8.1, 200 mM CaCl2