5W52

MicroED structure of the segment, DLIIKGISVHI, from the RRM2 of TDP-43, residues 247-257

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-01-19
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	24.810, 4.730, 15.830
Unit cell angles	80.88, 86.37, 89.78

Refinement procedure

Resolution	15.600 - 1.400
R-factor	0.2655
Rwork	0.262
R-free	0.30650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	idealized 11-residue beta strand
RMSD bond length	0.009
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.600	15.600	1.480
High resolution limit [Å]	1.400	4.430	1.400
Rmerge	0.203	0.138	1.451
Rmeas	0.213	0.142	1.562
Total number of observations	9353
Number of reflections	1037	22	139
<I/σ(I)>	3.34	7.45	0.92
Completeness [%]	73.4	59.5	62.1
Redundancy	9.019	9.864	6.712
CC(1/2)	0.992	0.998	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH	8.5	310	50 mM CHES, pH 8.5