5W50

Crystal structure of the segment, LIIKGI, from the RRM2 of TDP-43, residues 248-253

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-06
Detector	ADSC HF-4M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	11.543, 9.592, 21.175
Unit cell angles	95.63, 98.21, 76.50

Refinement procedure

Resolution	20.910 - 1.400
R-factor	0.1714
Rwork	0.168
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2omq
RMSD bond length	0.011
RMSD bond angle	1.643
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.450
High resolution limit [Å]	1.400	3.020	1.400
Rmerge	0.127	0.089	0.361
Number of reflections	1561
<I/σ(I)>	5.9
Completeness [%]	94.5	94.5	94.6
Redundancy	4.6	4.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	100 mM CHES, pH 9.5, 20% PEG8000, 10 mM lithium hydroxide