5W3U
Crystal structure of SsoPox AsB5 mutant (V27A-I76T-Y97W-Y99F-L130P-L226V)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.930, 105.950, 152.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.640 - 2.500 |
R-factor | 0.2152 |
Rwork | 0.212 |
R-free | 0.27170 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.640 |
High resolution limit [Å] | 2.500 |
Number of reflections | 47092 |
<I/σ(I)> | 12.84 |
Completeness [%] | 99.5 |
Redundancy | 4.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20-30 % (w/v) PEG 8000 and 50 mM Tris-HCl buffer (pH 8) |