5W38

1.80A resolution structure of human IgG3 Fc (N392K)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	64.429, 60.443, 71.396
Unit cell angles	90.00, 109.24, 90.00

Refinement procedure

Resolution	34.729 - 1.800
R-factor	0.1856
Rwork	0.183
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dz8
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.11_2568)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.729	45.000	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.055	0.027	0.575
Total number of observations	156654
Number of reflections	47374
<I/σ(I)>	12.7
Completeness [%]	98.4	97.8	97.7
Redundancy	3.3	3.3	3.2
CC(1/2)	0.998	0.999	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	20% (w/v) PEG 4000, 100 mM sodium citrate/citric acid, 10% (v/v) 2-propanol