5W35
Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with a double-stranded DNA oligomer with a 1-nucleotide overhang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-11 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.995, 210.414, 47.752 |
| Unit cell angles | 90.00, 108.60, 90.00 |
Refinement procedure
| Resolution | 35.000 - 3.310 |
| R-factor | 0.22617 |
| Rwork | 0.224 |
| R-free | 0.27857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w34 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 12200 |
| <I/σ(I)> | 24 |
| Completeness [%] | 99.8 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100 mM Hepes pH 7.0, 6% PEG 4000, 5 mM SrCl2 |
