5W2M
APOBEC3F Catalytic Domain Complex with a Single-Stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.194, 108.489, 108.549 |
| Unit cell angles | 79.60, 71.80, 71.75 |
Refinement procedure
| Resolution | 50.000 - 3.700 |
| Rwork | 0.250 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.700 |
| Number of reflections | 29144 |
| <I/σ(I)> | 4.7 |
| Completeness [%] | 96.2 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M HEPES, pH 7.0, 0.1 M Potassium chloride, 0.005 M Magnesium Sulfate, 15%(v/v) MPD |
