5W23
Crystal Structure of RSV F in complex with 5C4 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 183.360, 183.360, 275.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.060 - 3.400 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c69 4mms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.060 | 34.060 | 3.480 |
| High resolution limit [Å] | 3.400 | 15.580 | 3.400 |
| Rmerge | 0.380 | 0.073 | 1.596 |
| Rmeas | 0.406 | 0.081 | 1.707 |
| Rpim | 0.140 | 0.033 | 0.585 |
| Total number of observations | 470679 | ||
| Number of reflections | 64552 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 99.1 | 87.4 | 99.2 |
| Redundancy | 7.3 | 5.8 | 7.2 |
| CC(1/2) | 0.968 | 0.992 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Seed stock: 0.01 M zinc sulfate heptahydrate, 0.1 M MES hydrate pH 6.5, 22.5% v/v PEG MME 550 and 0.5% w/v polyvinylpyrrolidone K15 Reservoir solution: 0.2 M ammonium acetate, 0.1 M BIS-TRIS pH 6.5 and 25% PEG 3350 |
