5W1X

Crystal Structure of Humanpapillomavirus18 (HPV18) Capsid L1 Pentamers Bound to Heparin Oligosaccharides

Replaces:  3OFL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	80.993, 106.490, 237.661
Unit cell angles	88.46, 85.75, 69.02

Refinement procedure

Resolution	42.794 - 3.374
R-factor	0.174
Rwork	0.171
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r5i
RMSD bond length	0.004
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.794	3.829
High resolution limit [Å]	3.374	3.374
Rmerge	0.083	0.274
Number of reflections	92923
<I/σ(I)>	13.27
Completeness [%]	90.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	20% PEG3350, 0.2 M potassium acetate, pH 4.6