5W1V

Structure of the HLA-E-VMAPRTLIL/GF4 TCR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	71.646, 228.246, 276.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.930 - 3.310
R-factor	0.228
Rwork	0.225
R-free	0.26800
RMSD bond length	0.008
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.930	3.431
High resolution limit [Å]	3.312	3.312
Rmerge	0.056	0.278
Number of reflections	68257	6341
<I/σ(I)>	13.35	2.75
Completeness [%]	99.0	94
Redundancy	2	2
CC(1/2)	0.996	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M K, Na tartrate, 20% PEG 3350