5W1L

Echinococcus granulosus thioredoxin glutathione reductas (egTGR) with Gold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	109.346, 109.346, 258.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.720 - 2.880
R-factor	0.18786
Rwork	0.185
R-free	0.24898
RMSD bond length	0.019
RMSD bond angle	2.145
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.870
Number of reflections	36052
<I/σ(I)>	16.8
Completeness [%]	99.0
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	18% (w/v) polyethylene glycol 3350, 0.1 M lithium sulfate, 0.1 M Tris-HCl, pH 7.5