5W1B
Tryptophan indole-lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 62.785, 152.577, 205.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.580 - 2.000 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ax4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.751 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.580 | 2.167 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.225 | 4.666 |
| Rmeas | 0.233 | 4.829 |
| Rpim | 0.061 | 0.689 |
| Number of reflections | 133113 | 10672 |
| <I/σ(I)> | 9.97 | 0.46 |
| Completeness [%] | 97.2 | 92.33 |
| Redundancy | 14.7 | 13.9 |
| CC(1/2) | 0.998 | 0.327 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 35 mM potassium phosphate, 0.05 M HEPES, 0.3 M KCl, 11% PEG 4000 |
