5W1A

The first X-ray crystal structure of an insect muscle myosin. Drosophila melanogaster, skeletal muscle myosin II, an embryonic isoform, subfragment-1

Replaces:  4QBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	.9795
Spacegroup name	P 21 21 21
Unit cell lengths	108.554, 148.582, 148.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.117 - 2.227
R-factor	0.1805
Rwork	0.178
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.495
Data reduction software	XDS (NECAT)
Data scaling software	XDS (NECAT)
Phasing software	BALBES
Refinement software	PHENIX ((dev_2666))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	148.730	2.310
High resolution limit [Å]	2.227	2.230
Rmerge	0.090
Rmeas	0.116
Rpim	0.053
Number of reflections	116714
<I/σ(I)>	11	2.4
Completeness [%]	99.0	97.3
Redundancy	4.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	293	81% (0.1 M (0.03 M Citric acid, 0.07 BIS-TRIS propane, pH 7.1), 22% w/v PEG 3350), 9% Hampton Research Slice pH screen condition F9 (1.0 M TRIS hydrochloride, pH 8.1), and 10% Hampton Research Silver Bullet Bio screen condition E11 (0.2% w/v 1,2-Diaminocyclohexane sulfate, 0.2% w/v Diloxanide furoate, 0.2% w/v Fumaric acid, 0.2% w/v Spermine, 0.2% w/v Sulfaguanidine, 0.02 M HEPES sodium pH 6.8)