5W0T

Crystal structure of monomeric Msp1 from S. cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-30
Detector	PSI PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 32 1 2
Unit cell lengths	56.351, 56.351, 206.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.902 - 2.630
R-factor	0.2317
Rwork	0.230
R-free	0.25960
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.550
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	206.710	2.700
High resolution limit [Å]	2.630	2.630
Rmerge	0.211	1.447
Rpim	0.051	0.491
Number of reflections	11608	843
<I/σ(I)>	9.9	1.2
Completeness [%]	100.0	100
Redundancy	17.4	9.6
CC(1/2)	0.990	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	~9 mg/ml protein in 20 mM Hepes pH 7.5, 100 mM NaCl and 1 mM DTT was mixed with reservoir solution containing 16% PEG3350 and 0.6 M Sodium Thiocyanate