5W0M

Structure of human TUT7 catalytic module (CM) in complex with U5 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 61
Unit cell lengths	135.472, 135.472, 179.268
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	98.168 - 2.298
R-factor	0.1904
Rwork	0.188
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0b
RMSD bond length	0.005
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	179.000	2.310
High resolution limit [Å]	2.298	2.298
Rmerge	0.049	0.556
Number of reflections	82731
<I/σ(I)>	22.7
Completeness [%]	99.9
Redundancy	5.6
CC(1/2)	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.8-1.0 M lithium sulfate, 0.075-0.2 M potassium iodide, 0.05-0.2 M trisodium citrate, pH 5-6