5W0H

Structure of U2AF65 (U2AF2) RRM2 at 1.11 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9779
Spacegroup name	P 21 21 21
Unit cell lengths	33.430, 38.203, 72.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.690 - 1.110
R-factor	0.1537
Rwork	0.153
R-free	0.16810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2G4B RRM2
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.690	1.130
High resolution limit [Å]	1.110	1.110
Rmerge	0.081	0.278
Number of reflections	36104
<I/σ(I)>	27.8	4.5
Completeness [%]	97.2	92.2
Redundancy	5.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	26% (w/v) PEG 8000, 0.1 M NaOAc, 0.2 mM TCEP, 10% (v/v) dioxane, 0.2 ul BigCHAP, 0.05 M potassium phosphate monobasic