5W0G

Structure of U2AF65 (U2AF2) RRM1 at 1.07 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9779
Spacegroup name	P 43 21 2
Unit cell lengths	28.707, 28.707, 185.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.980 - 1.070
R-factor	0.136
Rwork	0.135
R-free	0.15300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2hzc
RMSD bond length	0.023
RMSD bond angle	1.393
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.980	1.090
High resolution limit [Å]	1.070	1.070
Rmerge	0.063	0.250
Number of reflections	33973
<I/σ(I)>	16.3	2.9
Completeness [%]	94.9	67.5
Redundancy	4.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	21% (w/v) PEG MME 550, 210 mM zinc acetate, 100 mM MES