5W0D

Inferred precursor (UCA) of the human antibody lineage K03.12 in complex with influenza hemagglutinin H1 Solomon Islands/03/2006

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 1
Unit cell lengths	40.620, 61.660, 83.860
Unit cell angles	106.57, 95.25, 106.70

Refinement procedure

Resolution	39.850 - 1.899
R-factor	0.198
Rwork	0.196
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5W0D & 5UGY
RMSD bond length	0.008
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.967
High resolution limit [Å]	1.899	1.899
Rmerge	0.112	1.395
Number of reflections	54618	5534
<I/σ(I)>	8.22	1.13
Completeness [%]	94.2	93.86
Redundancy	4.4	4.3
CC(1/2)	0.996	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	200 mM sodium chloride, 100 mM PIPES, pH 6.0, 20% w/v PEG2000 MME