5W0A

Crystal structure of Trichoderma harzianum endoglucanase I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-15
Detector	APEX II CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	74.585, 99.325, 114.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.821 - 2.898
R-factor	0.2382
Rwork	0.234
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eg1
RMSD bond length	0.020
RMSD bond angle	1.409
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11rc2_2531: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.821	3.100
High resolution limit [Å]	2.898	2.898
Rpim	0.175	0.726
Number of reflections	19374	3061
<I/σ(I)>	5.3
Completeness [%]	99.8	99.6
Redundancy	19.7	13.5
CC(1/2)	0.940	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.1 M ammonium sulfate and 0.05 M Tris-HCl pH 7.5