5W08

A/Texas/50/2012(H3N2) Influenza hemagglutinin in complex with K03.12 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999876
Spacegroup name	P 1 21 1
Unit cell lengths	60.800, 325.120, 156.990
Unit cell angles	90.00, 97.63, 90.00

Refinement procedure

Resolution	49.413 - 2.600
R-factor	0.241
Rwork	0.240
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4O5N 4wuk 4hkx 3tnn
RMSD bond length	0.006
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.275	2.635
Rmeas	0.297	2.840
Rpim	0.108	1.036
Number of reflections	182963	18367
<I/σ(I)>	8.29	1.19
Completeness [%]	99.2	99.78
Redundancy	7.5	7.1
CC(1/2)	0.985	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	100 mM sodium citrate/citric acid, pH 5.5, 20% w/v PEG3000