5W05
ANTI-TISSUE FACTOR ANTIBODY M59, A HUMANIZED VERSION OF 10H10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.380, 80.650, 112.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.640 |
| R-factor | 0.18751 |
| Rwork | 0.187 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m7k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.040 | 0.179 |
| Number of reflections | 45702 | 2034 |
| <I/σ(I)> | 38 | 5.7 |
| Completeness [%] | 93.1 | 56.4 |
| Redundancy | 8.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES PH 6.5, 16% PEG 3350, 0.2 M SODIUM FORMATE |
