5VZH

Post-catalytic complex of human Polymerase Mu (W434H) mutant with incoming UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.637, 68.722, 109.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.042 - 1.950
R-factor	0.1805
Rwork	0.179
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.007
RMSD bond angle	0.819
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rpim	0.023	0.332
Number of reflections	33554	1620
<I/σ(I)>	22.7	2.01
Completeness [%]	100.0	99.9
Redundancy	7	4.2
CC(1/2)		0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	90mM HEPES pH 7.5, 18% PEG4K