5VZ3
Growth Factor Crystal Structure at 1.97 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7430 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 49.964, 123.020, 41.698 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.700 - 1.970 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.24830 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 4.240 | 1.970 |
| Rmerge | 0.082 | 0.060 | 0.371 |
| Total number of observations | 384897 | ||
| Number of reflections | 9013 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 95.3 | 99 | 72 |
| Redundancy | 42.7 | 49.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 25% v/v MPD, 0.1 M sodium citrate, pH 4.0, 10 mM ATP |
