5VYV
Crystal structure of a protein of unknown function YceH/ECK1052 involved in membrane biogenesis from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-05-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.777, 74.853, 58.661 |
| Unit cell angles | 90.00, 100.73, 90.00 |
Refinement procedure
| Resolution | 28.817 - 2.480 |
| R-factor | 0.2094 |
| Rwork | 0.206 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u8j |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.092 | 0.806 |
| Rpim | 0.048 | 0.435 |
| Number of reflections | 11477 | 565 |
| <I/σ(I)> | 21.19 | 2.08 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 4.4 |
| CC(1/2) | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 8K, 50 mM potassium dihydrogen phosphate |
