5VYL

Crystal Structure of N-terminal half of Herpes Simplex virus Type 1 UL37 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979180
Spacegroup name	P 61 2 2
Unit cell lengths	138.232, 138.232, 164.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	96.737 - 3.510
R-factor	0.2442
Rwork	0.240
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k70
RMSD bond length	0.006
RMSD bond angle	0.933
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	164.220	3.850
High resolution limit [Å]	3.510	3.510
Rmerge	0.441	1.993
Rpim	0.119	0.539
Number of reflections	12161	2818
<I/σ(I)>	8.7	1.5
Completeness [%]	100.0	100
Redundancy	14	14.1
CC(1/2)	0.993	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	0.1M Bicine pH 9.0, 8% PEG 20K