5VXN

Structure of two RcsB dimers bound to two parallel DNAs.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	74.116, 113.231, 76.868
Unit cell angles	90.00, 115.37, 90.00

Refinement procedure

Resolution	56.615 - 3.375
R-factor	0.202
Rwork	0.199
R-free	0.26830
RMSD bond length	0.003
RMSD bond angle	0.556
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.230	3.390
High resolution limit [Å]	3.370	3.370
Rmerge	0.100	0.600
Number of reflections	15089
<I/σ(I)>	10.8	2.1
Completeness [%]	93.0	100
Redundancy	4.3	4.5
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Sodium Chloride, 0.1 M HEPES, 25% PEG3350