5VX5

VP8* of a G2P[4] Human Rotavirus in complex with LNFP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 21 21 21
Unit cell lengths	33.790, 50.240, 110.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.040 - 1.285
R-factor	0.1537
Rwork	0.153
R-free	0.16720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aen
RMSD bond length	0.006
RMSD bond angle	1.104
Data reduction software	iMOSFLM (7.0.9)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.040
High resolution limit [Å]	1.285	1.285
Rmerge	0.056	0.269
Rpim	0.022	0.102
Number of reflections	89379
<I/σ(I)>	19.58
Completeness [%]	93.4
Redundancy	2
CC(1/2)	0.999	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% w/v PEG4000