5VX3

Bcl-xL in complex with Bim-h3Pc-RT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	125.661, 68.811, 102.830
Unit cell angles	90.00, 125.35, 90.00

Refinement procedure

Resolution	41.936 - 1.945
R-factor	0.196
Rwork	0.194
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.844
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.936
High resolution limit [Å]	1.945
Rmerge	0.097
Number of reflections	52409
<I/σ(I)>	8.4
Completeness [%]	99.0
Redundancy	3.7
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		273	200 mM magnesium chloride, 20 % PEG 6000, and 100 mM sodium MES pH 6