5VX2
Mcl-1 in complex with Bim-h3Pc-RT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.031, 66.157, 76.673 |
| Unit cell angles | 90.00, 105.02, 90.00 |
Refinement procedure
| Resolution | 32.250 - 1.851 |
| R-factor | 0.1717 |
| Rwork | 0.169 |
| R-free | 0.21040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.761 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.250 |
| High resolution limit [Å] | 1.851 |
| Number of reflections | 34486 |
| <I/σ(I)> | 15.79 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 281 | 20 % PEG 3000, 100 mM trisodium citrate buffered with citric acid pH 5.5 |
