5VX0

Bak in complex with Bim-h3Glg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	48.092, 63.231, 121.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.693 - 1.599
R-factor	0.1663
Rwork	0.165
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.857
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.693
High resolution limit [Å]	1.599
Rmerge	0.156
Number of reflections	49543
<I/σ(I)>	11.78
Completeness [%]	100.0
Redundancy	9.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		281	200 mM magnesium chloride, 25 % PEG 3350, and 100 mM bis-tris chloride (pH 6.5)