5VWY

Bak core latch dimer in complex with Bim-h3Pc-RT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	65.890, 37.540, 69.560
Unit cell angles	90.00, 107.86, 90.00

Refinement procedure

Resolution	32.450 - 1.555
R-factor	0.1787
Rwork	0.175
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.138
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.450
High resolution limit [Å]	1.555
Rmerge	0.066
Number of reflections	21962
<I/σ(I)>	13.68
Completeness [%]	93.0
Redundancy	3.6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	15.8 % PEG 8000, 50 mM potassium dihydrogen phosphate, and 22.7 % glycerol