5VWI
Crystal structure of human Scribble PDZ1:Beta-PIX complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.869, 44.163, 60.465 |
Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
Resolution | 31.160 - 1.750 |
R-factor | 0.1983 |
Rwork | 0.194 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wyt |
RMSD bond length | 0.011 |
RMSD bond angle | 1.195 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.160 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.072 | 1.759 |
Number of reflections | 20310 | |
<I/σ(I)> | 17.6 | 1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.2 | 6.1 |
CC(1/2) | 0.999 | 0.307 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | 16.6% (w/v) polyethylene glycol 3350 and 0.1M citrate-bis-tris propane pH8.9 |