5VWG

Galectin-8 N terminal domain in complex with Methyl 3-O-[1-carboxyethyl]-beta-D-galactopyranoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-06-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	45.777, 50.145, 80.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.900 - 2.100
R-factor	0.2217
Rwork	0.220
R-free	0.25084
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t7u
RMSD bond length	0.007
RMSD bond angle	1.307
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.900	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.149	0.782
Number of reflections	11077	873
<I/σ(I)>	5.9	1.9
Completeness [%]	98.0	95
Redundancy	4.9	4.8
CC(1/2)	0.981	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate