5VW7
NADPH soak of Y316A mutant of corn root ferredoxin:NADP+ reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.188, 59.188, 188.032 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.454 - 1.608 |
| R-factor | 0.1332 |
| Rwork | 0.131 |
| R-free | 0.15560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lo8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.028 | 0.165 |
| Number of reflections | 50821 | 4336 |
| <I/σ(I)> | 24.5 | 6 |
| Completeness [%] | 98.6 | 86.4 |
| Redundancy | 4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |
