5VU6
TNA polymerase binary complex with primer/template duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.090, 110.800, 149.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.928 - 3.000 |
| R-factor | 0.2382 |
| Rwork | 0.235 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k8z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.748 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.930 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.091 | 0.779 |
| Number of reflections | 22536 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M MES, pH 6.0, 16% PEG3500, 0.2 M sodium sulfate, Silver Bullet Bio Additive #56 |
