5VU3
Crystal structure of the competence-damaged protein (CinA) superfamily protein ECL_02051 from Enterobacter cloacae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-02-28 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 44.529, 52.825, 132.156 |
Unit cell angles | 85.27, 87.19, 68.98 |
Refinement procedure
Resolution | 29.867 - 1.868 |
R-factor | 0.1521 |
Rwork | 0.151 |
R-free | 0.19280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kvk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.697 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2733)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.870 | 1.870 |
Rpim | 0.040 | 0.379 |
Number of reflections | 96710 | 4175 |
<I/σ(I)> | 26.33 | 2.63 |
Completeness [%] | 93.0 | 90.4 |
Redundancy | 2.7 | 2.7 |
CC(1/2) | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | PEG 2K MME, 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 10 mM nicotinamide mononucleotide |