5VTU
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225L/L226S mutant apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0331 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.554, 129.392, 72.894 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.450 |
| R-factor | 0.19913 |
| Rwork | 0.198 |
| R-free | 0.21956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 (712) |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rpim | 0.040 | 0.260 |
| Number of reflections | 66821 | 5155 |
| <I/σ(I)> | 18.2 | 1.6 |
| Completeness [%] | 98.0 | 83.3 |
| Redundancy | 6.3 | 4.4 |
| CC(1/2) | 1.000 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium nitrate and 20% PEG 3350 |
