5VTM
The crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.546, 76.760, 102.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.510 - 2.041 |
| R-factor | 0.1945 |
| Rwork | 0.191 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bw0 3ci0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.510 |
| High resolution limit [Å] | 2.000 |
| Rmeas | 0.052 |
| Rpim | 0.015 |
| Number of reflections | 31625 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 99.2 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 19 %-22 % PEG 2000 MME, 0.1M Tris, pH 8.0-9.0, 0.2 M trimethylamine N-oxide |
