5VSL

Crystal structure of viperin with bound [4Fe-4S] cluster and S-adenosylhomocysteine (SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	58.934, 73.854, 141.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.065 - 1.972
R-factor	0.1571
Rwork	0.155
R-free	0.19970
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	2.008
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX ((1.11.1_2575: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.079	0.578
Number of reflections	42331	4223
<I/σ(I)>	12.6	2.4
Completeness [%]	95.0	96.7
Redundancy	5.2	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	100 mM HEPES, pH 6.8 - 7.6, 10 - 30 % (w/v) PEG MME 2000, and 5 mM SAH