5VSH
CH1/Clambda Fab based on Pertuzumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-04-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.531, 106.207, 156.772 |
| Unit cell angles | 90.00, 117.48, 90.00 |
Refinement procedure
| Resolution | 25.700 - 2.550 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.700 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.138 | 0.527 |
| Number of reflections | 60826 | 8443 |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 93.1 | 89.2 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | see publication |
