5VRQ
Crystal structure of Legionella pneumophila effector AnkC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6307 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.705, 78.705, 156.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.689 - 3.205 |
| R-factor | 0.2257 |
| Rwork | 0.223 |
| R-free | 0.28040 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.318 |
| High resolution limit [Å] | 3.205 | 3.205 |
| Number of reflections | 8451 | 832 |
| <I/σ(I)> | 51.9 | 7.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 18.7 | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.25 M magnesium formate, 5 mM EDTA, 0.1 M sodium acetate |
